OPV疫苗感染性滴度检测的影响因素分析
Analysis of factors affecting detection of infectious titers of oral poliovirus vaccine
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摘要:目的 探索影响口服脊髓灰质炎减毒活疫苗(OPV)感染性滴度检测的主要因素。方法 采用96孔组织培养板微量细胞病变法进行感染性滴度检测, 分析对检测有影响的各种因素。结果 细胞浓度在5~40万个/ml时, 所测滴度差异不明显; 当细胞浓度在2.5万个/ml时, 所测滴度明显降低; 33.5℃及35.5℃温度下检测结果之间差异无统计学意义(P > 0.5);290~300代细胞所测滴度均低于150~160代细胞所测滴度, 差异有统计学意义(P < 0.001)。PolioⅡ+Ⅲ多克隆血清对Ⅰ型病毒的交叉抑制值为0.120±0.116;PolioⅠ+Ⅲ多克隆血清对Ⅱ型病毒的交叉抑制值为0.161±0.179;PolioⅠ+Ⅱ多克隆血清对Ⅲ型病毒的交叉抑制植为0.227±0.145。结论 温度及细胞浓度不是影响检测结果的敏感因素; 细胞代次是影响检测结果的敏感因素; 分型滴度检测时不同血清之间交叉抑制值的高低, 取决于病毒与血清之间的比例, 血清含量越高, 交叉抑制值越大。Abstract:Objective To assess the factors in the detection of infectious titers of oral poliovirus vaccine.Methods The cytopathogenic microtitration assay in 96 well plates were used in these experiments.Results Cell numbers ranging from fifty thousand to four hundred thousand showed no significant differences in infectious titers, but when cell number was under tweny five thousand the titers showed significant decrease.The infectious titers showed no significant differences at 33.5 and 35.5(P > 0.5).The infectious titers with cell passages of 290 through 300 were significantly lower than those with 150 through 160(P < 0.001).The cross inhibition of type Ⅱ and Ⅲ poliovirus polycolonal antibodies to type Ⅰ poliovirus was 0.120±0.116, the value of type Ⅰ and Ⅲ poliovirus was 0.161±0.179 for type Ⅱ, the value of type Ⅰ and Ⅱ was 0.227±0.145 for type.Conclusion Cell passage is an important factor.The cross inhibitions of different types of sera depend on the ratio of viral inoculation level and serum concentration.
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