Objective   To study the damage effect of inhalation carbon disulfide on germ cells of mice.

  Methods   The damage of germ cells of mice exposed to carbon disulfide by 2 h static inhalation per day at different concentrations(0, 200, 400, 800 mg/m³)for 5 weeks was analyzed with single cell gel electrophoresis(SCGE)and flow cytometry(FCM).

  Results   In four groups, the rate of DNA damage were 48.50%, 71.00%, 86.50% and 23.50% respectively, there was significant difference between the experiment group and control(P < 0.01);the percentage of head of the coment were(62.9949±8.6242)%, (56.6580±10.5990)%, (36.5432±12.0002)% and(86.8926±5.0219)% respectively; there was significant difference between the experiment group and control and every experiment groups(P < 0.001);the tail length in every groups were(8.6812±4.333), (14.0392±4.168), (28.6601±8.369)and(5.9913±1.924)m respectively, there was significant difference between middle, high dosage groups and control(P < 0.001).The rate of apoptosis in all groups were 12.53%, 14.93%, 29.09% and 11.97% respectively, and only high dosage group had significant difference compared with the control.

  Conclusion   The sub chronic carbon disulfide exposed can induce DNA damage of germ cells.