Abstract:
Objective To investigate the hazards of blowing sand and dust storm fine particulate matter(PM2.5)on rat alveolar macrophage membranes.
Methods The ambient PM2.5 samples were collected on normal, blowing sand and dust storm days in Wuwei city, Gansu province and Baotou city, Inner Mongolia Autonomous region.After rat alveolar macrophages(AM)were treated 4 h with particle suspensions of the samples, cytotoxicity was assessed using methyl thiazolyl tetrazolium(MTT)reduction assay.Also, activities of plasma membrane Ca2+ Mg2+-AT Pase and Na+ K+-AT Pase, activties of lactate dehydrog enase(LDH)and acid phosphatese(ACP)in culture meduim, and plasma membrane fluidity were examined.
Results Both the blowing sand and dust storm PM2.5 could decrease cell viability, inhibit plasma membrane Ca2+ Mg2+-AT Pase and Na+ K+-AT Pase, increase leakage of cytosolic LDH and ACP, and elevate surface fluidity of plasma membranes in a dose-dependent manner as compared with the control saline.And the two-way ANOVA showed there was no significant difference on alterations of the measured indices between normal, blowing sand and dust storm groups.
Conclusion Blowing sand and dust storm PM2.5 could make adverse effects on AM by increasing plasma membrane permeability and fluidity, leading to cytotoxicity.It suggested that appropriate attention should be focused on their to xicities.