野生多汁乳菇的抗辐射作用

刘佳; 高敏; 向红; 俞红; 阮海星; 吴克枫; 王雨

doi:10.11847/zgggws2006-22-04-47

野生多汁乳菇的抗辐射作用

Antiradiation effect of wild lactarius volemus on mice

摘要

摘要:
目的观察贵州野生多汁乳菇对小鼠的抗辐射作用。
方法设3个多汁乳菇剂量组2.3, 4.5, 9.0g/(kg·bw)、阴性对照组和辐射模型对照组, 辐射剂量为5 Gy。观察小鼠白细胞计数、测定骨髓DNA含量、骨髓微核率3个指标。
结果辐照后5 d各剂量组小鼠的白细胞总数明显高于辐射对照组(P < 0.01);辐照后4, 15 d, 多汁乳菇各剂量组小鼠DNA损伤随剂量的增加, 损伤程度逐渐降低, 呈剂量-反应关系(P < 0.05);多汁乳菇9.0 g/(kg·bw)组在辐照4 d后骨髓微核率较辐射对照组有明显降低(P < 0.01)。
结论在本实验条件下, 多汁乳菇对辐射导致的小鼠白细胞损伤、DNA损伤有一定的保护作用, 在剂量为9.0 g/(kg·bw)时, 对染色体受损有良好的保护作用。

Abstract:
Objective To observe the antiradiation effect of wild lactarius volemus on ince in Guizhou province.
Methods Mice were randomly divided into 5 groups: 3 test groups administered with lactarius volemus2.3, 4.5, 9.0 g/(kg·bw)respectively, negative control group, and positive control group.Mice were dealt with 5 Gy irradiation in test and positive groups.The numbers of peripheral blood leukocytes, the contents of bone marrow DNA, and the poly chromatic erythrocytic (PE)micronucleus formation rate of bone marrow were detected.
Results The numbers of peripheral blood leukocytes in test groups were sig nificantly more than that in positive control group on day 5 after irradiation(P > 0.01);there were negative correlation between the dose of lactarius volemus and the level of DNA damage and had dose-effect relationship on days 4, 15 after irradiation(P < 0.05).Micronucleus rate in the high dose group9.0 g/(kg·bw)were significantly lower than that in the positive control on days 4 after irradiation(P < 0.01).
Conclusion The wild lactarius volemus protects the mice from the damage of leukocytes and DNAinduced by irradiation.In the 9.0 g/(kg·bw)group, wild lactarius volemus is of a better protection against the damage of chro mosome induced by irradiation.

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