Abstract:
Objective To explore the alteration of target molecular sequences in the noncoding mitochondrial DNA(mtDNA)contr ol region in 16HBE cells transformed by carcinog enstrans-BPDE and crystalline nickel sulfide.
Methods The sequences of mtDNAcontrol region in 16HBE cells were detected by PCR-SSCP assay and sequencing.
Results Compared with those of control 16HBE cells, no change in hypervariable segments of mtDNA in malignant transformed cells and tumorigenic cells induced by trans-BPDE and crystalline nickel sulfide was detected.
Conclusion The noncoding mtDNA control region might not be a target sequence in carcinog enesis induced by chemical carcinogen.