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安社娟, 陈家堃, 常薇, 陈华洁, 刘莉莉, 赵艳丰, 陈学敏. 层粘连蛋白受体基因表达谱分析[J]. 中国公共卫生, 2006, 22(8): 932-933. DOI: 10.11847/zgggws2006-22-08-25
引用本文: 安社娟, 陈家堃, 常薇, 陈华洁, 刘莉莉, 赵艳丰, 陈学敏. 层粘连蛋白受体基因表达谱分析[J]. 中国公共卫生, 2006, 22(8): 932-933. DOI: 10.11847/zgggws2006-22-08-25
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AN Shejuan, CHEN Jiakun, CHANG Wei, . Analysis on gene expression profile of laminin receptor[J]. Chinese Journal of Public Health, 2006, 22(8): 932-933. DOI: 10.11847/zgggws2006-22-08-25
Citation: AN Shejuan, CHEN Jiakun, CHANG Wei, . Analysis on gene expression profile of laminin receptor[J]. Chinese Journal of Public Health, 2006, 22(8): 932-933. DOI: 10.11847/zgggws2006-22-08-25
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层粘连蛋白受体基因表达谱分析

Analysis on gene expression profile of laminin receptor

    摘要
  • 摘要:
      目的   探讨层粘连蛋白受体(67LR1)基因相关的一组基因表达变化, 为反式二羟环氧苯并芘致癌机制的研究提供依据。
      方法   构建67LR1转基因表达载体, 转染人支气管上皮细胞(16HBE), 获得转基因67LR1的瞬时表达的细胞株。用半定量RT-PCR方法分析转基因表达产物, 应用含8 064个基因点的微芯公司基因表达谱芯片CSC-GE-80与双荧光标记样品与芯片杂交和扫描、图像数据分析等一系列处理进行表达谱分析。
      结果   成功构建出瞬时表达67LR1的细胞株。基因表达谱分析, 2组相比发生了显著性表达变化的基因有295个, 其中表现为上调的基因有145个, 下调表达的基因有150个。在发生了显著性表达改变的基因中, 比较有明显功能分类特征的包括与信号转导相关的基因, 与肿瘤相关的基因, 与免疫反应相关的基因以及与蛋白质合成系统相关的基因等。
      结论   67LR1基因功能涉及到信号转导、肿瘤相关基因等方面, 相关基因的表达变化具有复杂性。

     

    Abstract:
      Objective   To investigate the gene expression changes related to laminin receptor, and to give the basis data for the study of carcinog enesis of anti-BPDE.
      Methods   The transient transfected 16HBE cell line with 67LR1 gene was constructed by using lipofectin protocols.The expression products of transfected genes was analyzed by semi-quantity RT-PCR.The gene expression patterns in the transient transfected 67LR1 cell line and the non-transfected parental cell line were detected by cDNA microarray which representing 8 046 different genes.
      Results   The transient 16HBE cell lines with 67LR1 was successfuly constructed.Analysis of the gene expression profile indicated that there were 295 genes expressed differently between transfected 67LR1 gene lines and the non-transfented parental cell line.Among these changed genes, 145 genes were up-regulated, and 150 genes down regulated.These significantly changed genes involved in cell signal transduction genes, tumo-rrelated genes, immune-related genes and protein synthesis related genes.
      Conclusion   The analysis of 67LRl-related gene profile alterations indicate that 67LR1 may be induces not by an alteration in the expression of a single gene and a single signal pathway, but by the accumulation of alteration in the expression of several genes and complex signal transduction network.

     

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