大气混合污染物对大鼠呼吸系统氧化损伤作用

肖纯凌; 王任群; 赵肃; 金焕荣; 王灿

doi:10.11847/zgggws2006-22-08-27

大气混合污染物对大鼠呼吸系统氧化损伤作用

Oxidized injury of respiratory system in rats exposed to air pollutants

摘要

摘要:
目的探讨大气混合污染物所致大鼠呼吸系统的氧化损伤。
方法 48只Wistar大鼠随机分为3个实验组(1, 7, 30 d组)和1个对照组。气管注入可吸入颗粒物(PM10)后, 静态吸入SO₂、NO₂、CO的空气混合气, 分别吸入1, 7及30 d。检测血清和支气管肺泡灌洗液(BALF)中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力及谷胱甘肽(GSH)、丙二醛(MDA)的含量。
结果 1 d组对与对照组比较, 血清SOD活力显著降低(P < 0.05), GSH和MDA含量显著增高(P < 0.01);30 d组与对照组比较, BALF中SOD、GSH-Px活力及GSH含量显著降低(P < 0.05或P < 0.01)。
结论大气混合污染物对大鼠呼吸系统产生氧化损伤作用。

Abstract:
Objective To explore the oxidized injury of respiratory system in rats exposed to air pollutants.
Methods 48 Wistar rats were divided into three treated groups(1, 7, 30 d)and one control group randomly.The treated rats were exposed to PM 10 by intratracheal instillation, and then were exposed to mixed gases of SO₂, NO₂, and CO by static inhalation for 1, 7, and 30 d.The activities of superox ide dismutase(SOD)and glutathione pero xidase(GSH-Px)and the levels of GSH and malondialdehy de(MDA)in serum and BALF were measured.
Results The activity of SOD in serum was significantly decreased, and the levels of GSH and MDA in serum were significantly increased in 1 d group compared with control group.T he activities of SOD and GSH-Px and the levels of GSH in BALF of rats in 30 d group were significantly lower than those in control group.
Conclusion Air pollutants can induce the ox idized injury of respiratory system in rats.

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