有机污染物对人肝肿瘤细胞的遗传毒性

李晓燕; 刘爱林; 鲁文清

doi:10.11847/zgggws2006-22-08-37

有机污染物对人肝肿瘤细胞的遗传毒性

Genetic toxictity of three POPs on human derived hepatoma cells

摘要

摘要:
目的用人肝肿瘤细胞Hep G2/体外微核试验检测3种持久性有机污染物(POPs)Aroclor1254、毒杀芬和滴滴涕的遗传毒性。
方法人肝肿瘤细胞Hep G2经Aroclor1254、毒杀芬和滴滴涕染毒24 h, 继续在补充细胞松弛素B(3μg/ml)的培养液中培养24 h后, 计数1 000个双核细胞中的微核。
结果 20, 40μmol/L毒杀芬处理Hep G2细胞的微核率与溶剂对照相比显著增加(P < 0.01, P < 0.01);经Aroclor1254(23~184μmol/L)和滴滴涕(17.8~60μmol/L)处理的Hep G2细胞的微核率与溶剂对照相比, 差异无统计学意义。
结论 Aroclor1254和滴滴涕未显示对Hep G2细胞明显的遗传毒性; 毒杀芬可诱导Hep G2细胞遗传损伤, 有必要进一步评价其对人类健康的潜在危害。

Abstract:
Objective To investigate genetic toxicity of three persistent organic pollutants(POPs), including aroclor1254, DDT and Toxaphene, using human derived hepatoma(HepG2)cells/in vitromicronucleus assay.
Methods HepG2 cells were treated with Aroclor1254, DDT and Toxaphene respectively for 24 hours, and further incubated in the medium supplemented with cytochalasin B(3 μg/ml)for 24 hours.Micronuclei(MN)were scored in 1 000 binucleated Hep G2 cells.
Results Compared with solvent control, tox aphene increased significantly MN frequencies in HepG2 cells at concentrations of 20, 40 μmol/L respectively(P < 0.01, P < 0.01).No significant increase of MN frequencies were found in HepG2 cells treated with Aroclor1254(23~184 μmol/L)and DDT(17.8~60μmol/L).
Conclusion Aroclor1254 and DDT do not show clear genetic toxicity in HepG2 cells.Toxaphene could induce genetic damage in HepG2 cells, and further study is needed to evaluate its potential adverse effects on human health.

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