Abstract: Objective To study the effect of grape procyanidins(GPC)on the pro tein kinase C(PKC)expression in mice's hepatic cells in viro.Methods The nomal mice's hepatic cells were cultured with different dose of GPC.The proliferation activity of the hepatic cells was determined by MTT assay,and the levels of PKC protein expression in the cell were measured by immunocytochemistry method.Results The expression rates of PKC protein and the proliferative activity of 100 mg/L GPC group were 34.24% and 0.654±0.062,which were significantly higher than those of the control group by χ² test and t test(P<0.05).Conclusion GPC can promote the PKC protein expression and improve the proliferative activity of the mice's hepatic cells.