高级检索
姚苹苹, 徐芳, 朱函坪, 翁景清, 陆群英, 谢荣辉, 朱智勇. 汉坦病毒Vero细胞灭活疫苗候选毒种筛选[J]. 中国公共卫生, 2007, 23(2): 208-209. DOI: 10.11847/zgggws2007-23-02-45
引用本文: 姚苹苹, 徐芳, 朱函坪, 翁景清, 陆群英, 谢荣辉, 朱智勇. 汉坦病毒Vero细胞灭活疫苗候选毒种筛选[J]. 中国公共卫生, 2007, 23(2): 208-209. DOI: 10.11847/zgggws2007-23-02-45
shu
YAO Ping-ping, XU Fang, ZHU Han-ping, . Selection of virus strain for production of inactivated hantavirus vaccine of vero cells[J]. Chinese Journal of Public Health, 2007, 23(2): 208-209. DOI: 10.11847/zgggws2007-23-02-45
Citation: YAO Ping-ping, XU Fang, ZHU Han-ping, . Selection of virus strain for production of inactivated hantavirus vaccine of vero cells[J]. Chinese Journal of Public Health, 2007, 23(2): 208-209. DOI: 10.11847/zgggws2007-23-02-45
shu

汉坦病毒Vero细胞灭活疫苗候选毒种筛选

Selection of virus strain for production of inactivated hantavirus vaccine of vero cells

    摘要
  • 摘要: 目的 将肾综合征出血热疫苗后备筛选毒株适应于Vero细胞,并对其抗原性和免疫原性进行研究。方法 将新分离的4株汉坦病毒接种敏感动物,在乳沙鼠脑内连续传代,比较脑内病毒抗原的含量以及乳鼠的发病情况;在Vero细胞中传代,比较培养液中的病毒滴度和抗原滴度以及细胞的病变情况;利用筛选出的毒株研制Vero细胞灭活疫苗,研究疫苗的免疫原性。结果 4株病毒经乳沙鼠脑内传代,鼠脑悬液的病毒滴度和抗原滴度分别达7.00~7.75 LgCCID50/ml和1:320~1:1 280;4株毒株经Vero细胞连续传5代,培养液病毒滴度和抗原滴度分别达6.50~7.50 LgCID50/ml和1:160~1:768;疫苗免疫家兔2针后,其中出血热病毒株ZJ4与ZJ5株疫苗免疫血清对同型毒株的中和效价达到1:10。结论 ZJ4与ZJ5两毒株已适应于Vero细胞,且具有病毒滴度高和免疫原性良好的特性,适合用作Vero细胞肾综合征出血热疫苗理想的候选毒株。

     

    Abstract: Objective To adapt the candidate strains of hemorrhagic fever with renal syndrome(HFRS)vaccine to Vero cells and to study their antigenicity and immunogenicity.Methods 4 strains of Hantavirus were continuously propagated in suckling mouse brains or in Vero cells and studied the antigenicity of virus,viral titers,the amounts of virus antigen and their immunogenicity.Results Four strains of Hantavirus adapted to Vero cells and stably grew in the cells or in suckling mouse brains with high titers.Rabbits immunized with the crude vaccines of ZJ4 and ZJ5 showed the neutralizing antibody titers of the rabbit immune sera reached 1B10 at 4 weeks after 2 times of immunization.Conclusion ZJ4 and Z J5 candidate strains adapted to Vero cells,possessed high titers with good immunogenicity and were feasible to prepare the HFRS vaccine of Vero cells.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回