Abstract: Objective To explore the relationship between LHBs and HBV DNA,to study the reliability of LHBs reflecting HBV replication and to discover the clinical significance of detecting LHBs in HBeAg negative CHB patients by examing LHBs,HBV DNA and HBeAg in sera of CHB patients.Methods LHBs and HBeAg were detected by enzyme-linked immunosorbent assay(EL ISA)while HV DNA by quantitative fluorescent PCR.Results LHBs of 171 CHB patients were not statistically different from HBV DNA(P>0.05).The HBV DNA of different HBVM patterns had no statistical significance from LHBs(P>0.05)at the same time.And the logarithmic value of HBV DNA copies correlated with contents of LHBs(r=0.917).Conclusion LHBs,which might reflect HBV replication and correlated closely with HBV DNA,would be a novel innumo-serological index that might probably reflect the HBV replication of HBeAg-negative HBV patients.