Abstract: ObjectiveTo measure the level of interleukin 10 in the spleen tissue homogenate and in sera of guinea pigs and its significance.Methods28 FMMU guinea pigs were randomly divided into experimental group,negative control group and positive control group and then trichlor oethylene were applied on the skin of guinea pigs.IL-10 in the spleen tissue homogenate and sera was detected by enzyme-linked immunosor bert assays.ResultsThe level of IL-10 of experimental group in the spleen tissue homogenate and sera was significantly higher than that of the positive control groups and negative control group.ConclusionIL-10 may play a important role in allergy contacting dermatitis.