Abstract: ObjectiveTo observe the effect of chronic lead contaminant on protein expression of phosphory lated cAMP response element binding protein(pCREB)in hippocampus of mice.MethodsChronic acetic lead contaminant was applied on mice(214,4.8,9.6 mmol/L),and pups were killed at 14,28,42,56d respectively.Western blotting was used to observe protein expression of pCREB in hippocampus of mice.ResultsCompared with control group,pCREB protein expression of chronic lead exposure mice in the hippocampus was downtrend,2.4 mmol/L lead exposure could make the protein expression at 14 days higher than that of control(P>0.01),but at follow days lower than that of control.As to 4.8 mmol/Land 9.6 mmol/L concentration,the protein expression was always lower than that of control(P<0.05).ConclusionChronic lead exposure can disturb the normal change of pCREB protein expression in mice.