Abstract: Objective To evaluate prevalence of and risk factors for human immunodeficiency virvs(HIV)co-infection with hepatitis C virus(HCV)and hepatitis B virus(HBV)in rural commer cial blood donation area in Shanxi province,China.Methods A cross-sectional study was conducted and all residents aged 18~59 years in four villages in a county of Shanxi province were invited for questionnaire inter view and testing of HIV,HCV antibody and hepatitis B virus surface antigen(HB-sAg).Results A total of 3 062 villagers participated in this study and provided specimens for related testing.Seroprevalence of anti-HIV anti-HCV and HBsAg were 1.3%(40/3 062),12.7%(389/3 062)and 3.5%(103/2 982),respectively of the 40 anti-HIV seropositive participants,85.0% were anti-HCV seropositive,2.5% were HBsAg seropositive.History of commercial blood donation was positively associated with anti-HIV seropositivity anti-HCV seropositivity and HIV/HCV co-infection(all HIV/HCV co-infected participants were blood donors),but were negatively associated with HBsAg seropositivity.Migrated for work in last 5 years was positively associated with HIV,HBsAg seropositivity and HIV/HCV co-infection.Univariate analysis showed illegal drug use,number of sex partners,extramarital sex behavior,commercial sex behavior and condom use rate didn't associated with anti-HIV,anti-HCV HBsAg seropositvity and their co-infection.Conclusion History of commercial blood donation was main risk factor for HIV and HCV infection in this former commercial blood donation area.