Abstract: Objective In order to provide the base for choosing antibiotic empirically for each ward,the profile of the bacteria was investigated in the ICU ward and non-ICU ward and compared the rate of the antibiotic resistance to the gram negative bacilli.Methods The bacteria were identified by VITEK-2 and API system.Antibiotic sensitivity test adopted Kir-by-Bauer method.Data was analyzed by WHONET 5.3 software and SPSS 1115.Results The first five organisms in ICU ward were Pseudomonas aeruginosa(128 isolates,accounted for 22.1%),Burkholderia cepacia(59 isolates,10.2%),coagulase negative staphylococcus(50 isolates,8.6%),Staphylococcus aureus(39 isolates,6.7%)and Candida albicans (37 isolates,6.4%).In non-ICU ward the first five organisms were Escherichia coli(358 isolates,account for 16.4%),P.aeruginosa(296 isolates,13.6%),coagulase negative staphylococcus(194 isolates,8.9%),Klebsiella pneu moniae(188 isolates,8.6%)and S.aureus(184 isolates,8.5%).The antibiotic resistant rates of P.aeruginosa and E.coli from ICU ward were much higher than those in non-ICU ward.Conclusion Non-fermn,gram negative bacilli,are the main isolates in ICU ward and have more significantly high degree of antibiotic resistant rates than those in non-ICU ward,which are treated difficultly.So the antibiotic resistant rates in ICU ward should be monitored alone.