Abstract: Objective To explore the effect and mechanism of lotus-seed milk fermented production(LMFP)on the in testinal mucosal barrier of mice.Methods 60 male ICR mice were randomly assigned into five groups.The mice were pretreated intragastrically with distilled water,lotus-seed milk fermented production,lotus-seed milk and fermented milk once daily for two weeks.Twenty-four hours after the last dosing,animals were administered with LPS6 mg/(kg·bw)in traper itoneally,control animals were given distilled water only.Eighteen hours after the intoxication,the indices were measured.Results The mice injected with LPS showed the symptoms of sluggish in varying extent,especially in model group.The LMFP group was the most slight compared with other groups.The lotus-seed milk fermented production can signifi cantly improve the concentration of intestinal SIgA and the quantity of white blood cell(P<0.001,P=0.001),retain the amount of lactobacillus acidophilus(P<0.001),decrease the concentration of TNF-in serum induced by LPS and the quantity of Escherichia coli in the mouse's caecum.The protective effect of lotus-seed milk fermented production on the in testinal mucosal barrier harmed by LPS was significantly better than fermented milk,and there was no protection in lotusseed milk group.Conclusion The model of intestinal mucosal barrier harmed by LPS was established successfully.The lotus -seed milk fermented production can protect the intestinal mucosal barrier againstharm caused by LPS,and relieve the response of systemic inflammatory.