Abstract: Objective To observe the effect of disinfection of air in classroom and train carriage with electrolyzed oxidizing water(EOW)and deter mine the available dose and frequency of the disinfectant in disinfection process.Methods The concentration of the disinfectant used was 15mg/L(15ml/m3)in classroom and 10 mg/L(20 ml/m²)in train carriage respectively.The number of bacteria colonies at various time after disinfection was detected and the killing rate of bacteria was calculated.Results After disinfected,the number of bacteria in the air of classroom and train carriages descended gradually.After 80min disinfection,the number of bacteria in the air of the classroom was the least and the killing rate was the highest (90.7%),then the number of bacteria was slowly rising.After disinfected,the number of bacteria in the air of train carriage tended to descend,but affected by fluxion of passengers.Conclusion EOW has strong effect for disinfecting bacteria in the air of public rooms.After disinfected,the air hygienic quality in classroom and train carriage can reach hygienic standard of China.EOW can be used to disinfect the air in public rooms.