Abstract:
Objective To isolate,identify and purify the allergens in cashewnut,anacard lun occidentale L.
Methods The total protein of the cashew nutwas iso lated by SDS-PAGE.The allergens of cashew nutwere identified by Westernblotting.To purify the allergens,ion exchange chromatography was employed.
Results There were tenprotein bands visible on the SDS-PAGE gel.The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting.The 21kD and 11kD allergens of cashew nutwere purified by ion exchange chromatography.
Conclusion The allergens of cashewnut were isolated and identified.And the allergens of cashewnut were purified prmiarily.