Abstract: Objective To explore the distribution of VDR FokⅠ genotype in children who 1ived in a reas with or without high fluoride,and to evaluate the relationship between childrens dental fluorosis and VDR FokⅠ gene polymorphism.Methods Children aged 8 to 12 years,born and raised in high fluoride areas and contro1 areas in two counties of Henan province were recruited.They were divided into three groups according to dental fluorosis status:dental fluorosis group,non-dental fluorosis group from high fluoride area,and contorl group.The FokⅠ marker of VDR gene was genoty ped using PCR-RFLP procedure.Fluoride levels in urine samples from the three groups were detected by fluoride ion selective electrode method.Resu lts T he preva lence rate of dental fluorosis in high fluo ride areas was 51 7%.No dental fluorosis case was found in control areas.The frequency distribution of VDR FokⅠ genotype was FF 32.4% (24/74),Ff 45.9% (34/74),ff 21.6% (16/74) in children with dental fluorosis,FF 40.6% (28/69),Ff 36.2% (25/69),ff 23.2% (16/69) in children withoutdental fluorosis from high fluoride areas,and FF 31.9% (30/94),Ff 50.0% (47/94),ff 18.1% (17/94) in the children from contro1 areas,respectively.There was nosignificant difference among the three groups (P > 0.05).Conclusion There was no correlation bewteen dental fluorosis and VDR FokⅠ polymorphism.