Abstract: Objective To understand the status of health knowledge and attitude and to evaluate instant effect of education course among undergraduates in a university in Guangzhou.Methods Cluster random sampling was used to select 198 students participating a health education course and 293 students being control group.The two groups completed a selfadministered questionnaire on health knowledge and attitude.Results The average score of health know ledge in the control group(63.98±15.68) was much low er than that of the intervention group(81.17±12.84,t=13.30; P=0.000).Of all undergraduates,94.7% considered that it was necessary to develop a health education course on campus; 97.5% reported acquiring some knowledge from the course; 98.5% were satisfied with the content of the course.Of all undergraduates,84.6% did not took the course because they did not know the establishment of the course.Conclusion The undergraduates had poor health know ledge.It is necessary to develop a health education course to improve undergraduates ' health know ledge.