Abstract: Objective To test and apply a question bank for school-based HIV/AIDS education in junior high school students,and to explore its role in the supervision and evaluation on health education.Methods A total of 889 students from 10 junior high schools in Shanghai city,Guangdong and Sichuan province were sampled to test and modify the question bank.A preliminary study among 2 098 students from 14 junior high schools in 7 provinces was carried out with convenient cluster sampling to modity the question bank for school-based HIV/AIDS education.Results Results of parallelism and discrimination study on the question bank among the students in 16 classes showed that the difference betw een the tw o questionnaires compiled based on the bank was not statistically significant in 13 classes(P> 0.05) except for 1 class in Shanghai with significantly lower scores for the questionnaire 1(81.00) than that of the questionnaire 2(85.52) and 2 classes in Sichuan with signiticantly higher scores for the questionnaire 1(78.56,83.86) than that of questionnaire 2(72.52,78.68).The differences in the scores among the classes with the education program were significantly different from those of without the education.Students'achievements(75.06-87.48 scores) in schools with the health education were better than those in the schools with ordinary health education(72.52-83.20 scores) (P< 0.01).The overall score of the students in project schools in 7 provinces(72.17) was higher than that of students in non-project schools(68.40).The awareness rate of basic HIV/AIDS knowledge of the students in project schools(78.5%,1 104/1 407) was higher than that of the students in nonproject schools(71.8%,496/691,P< 0.01).Conclusion Questionnaires compiled based on the question bank have a good comparability and discrimination and could be used as a test in supervision and evaluation on HIV/AIDS education in schools.