Abstract: Objective To study the sleep quality and its influencing factors among university students in Guiyang city.Methods With stratified sampling,2 126 students from three universities were selected and surveyed with Pittsburgh Sleep Quality Index(PSQI) and a self-developed questionnaire on influencing factors of sleep quality.Multiple linear regression was used to analyze related factors of sleep quality.Results The average PSQI score was 6.27±2.34 and 27.4% of the students reported poor quality of sleep.There was no significant difference between the male and the female in the quality of sleep.The junior students had the worst sleep quality.Factors influencing the quality of sleep in the students included frequent web surfing(β=0.072),bad relationship between the father and mother(β=-0.049),having rocky relationship with teacher(β=-0.049),without regular physical excercise(β=-0.054),having bad relationship with classmates(β=-0.058),stress of study(β=-0.066),a failure in love(β=-0.069),neglect of self-health(β=-0.084),without good relationship with family members(β=-0.089),and worring about self-health constantly(β=-0.162).Conclusion The sleep quality is not good in university students of Guiyang city and specific measures should be taken for related factors to improve the quality of sleep.