幽门螺杆菌与人巨噬细胞株作用蛋白组学比较

Proteomic analysis of differential proteins in interaction of Helicobacter pylori and U937 cell line

摘要: 目的寻找幽门螺杆菌(Hp)与宿主细胞(U937)作用后的差异蛋白。方法利用双向电泳技术分离Hp26695和TN2株与U937细胞相互作用前后的蛋白,应用ImageMaster 2Dv3.1软件对蛋白图谱进行比较,对目的蛋白进行基质辅助激光解析及电离飞行时间质谱分析,应用Mascot软件进行蛋白图谱搜库比对。结果 2株作用后的细胞株共检测到2个差异蛋白斑点,经质谱分析鉴定与Mascot搜库证实,其中的1种差异蛋白为Hp热休克蛋白60。结论热休克蛋白60可能进入细胞或结合于宿主细胞膜上,其相关机制尚待进一步阐明。

Abstract: ObjectiveTo search for differential proteins after the interaction of Helicobacter pylori(Hp)and host cells (U937).MethodsTwo Hp strains(26695 and TN2)and cell line U937 were used.Two-dimensional electrophoresis (2-DE)and mass spectroscopy were applicated to separate protein and to identify differential protein.The protein maps were then compared by ImageMaster 2Dv3.1.ResultsTwo differential protein spots were found in the gel of 2-DE and the one was identified as heat shock protein 60(Hsp60).ConclusionHp Hsp60 could enter the host cell or connect to the membrane of the host cell and the mechanism needs to be explored.