Abstract: Objective To investigate risk factors and genotype of human papilloma virus(HPV) among female outpatients in Guangzhou city.Methods The female outpatients visiting the Third Affiliated Hospital of Sun Yat-sen University and receiving HPV test were selected from March 2010 through January 2011.A total of 360 female outpatients were checked for 21 HPV DNA types with DNA flow-though hybridization genotyping technique and a questionnaire survey was conducted among the women.Results The HPV infection rate in the women was 38.3%.Totally 19 types of HPV were identified including strains of 15 HPV-H and 4 HPV-L.HPV42 and HPV43 were not detected.HPV16,58,52,33 were the most prevalent high-risk subtypes.Low risk type HPVcp8304 was prevalent.The total infection rate of HPV18,6,11 was 2.5%.High-risk types were more frequently found in the outpatiens,with a infection rate of 34.4%.The infection rate of low-risk subtypes was 6.4% and the rate of multiple infection was 8.3%.No correlation between HPV infection and age was observed.The results of logistic regression showed that early age at first sexual intercourse(≤19 years old) and more than one sexual partner of the spouse were the independent risk factors of HPV infection.Conclusion HPV16,58,52 are the most prevalent high-risk subtypes and early age at first sexual intercourse and more than one sexual partner of the spouse are the independent risk factors of HPV infection among women in Guangzhou city.