Abstract: Objective To investigate the prevalence and trend of Japanese encephalitis(JE) during 1960-2010 in Guangxi Zhuang Autonomous Region and to provide scientific evidence for effective control and prevention of JE.Methods The data on JE epidemic in Guangxi from 1960-2010 were collected and analyzed with descriptive epidemiologic methods.Results Totally 65 384 people in the province suffered from JE during the period.Among of them,11 875 died with an average annual morbidity of 3.38/100 000,an average yearly mortality of 0.61/100 000,and a fatality rate of 18.2%.The epidemic peak occurred in 1960's and 1970's,with yearly average morbidities of 8.59/100 000 and 9.24/100 000,respectively.The disease incidence rate decreased significantly after the period.The JE cases were found in all 14 cities of Guangxi,among which the Guigang,Fangchenggang,Beihai,Laibin,and Yulin were cities with the higher morbidity of 5.40,4.12,4.02,3.88,and 3.81per 100 000 person years,respectively,accounting for 14.3%,1.9%,3.4%,5.7%,and 12.5% of total cases in the province,respectively.There was a distinct seasonal peak for the epidemics,mainly in the months of May to September,accouning for 95.8% of total cases.The cases occured mainly in the children under the age of 14 years with a higher incidence rate in male than in female.Conclusion The incidence of JE is decreased.The incidence of JE is sporadic with distinct seasonal peak and mainly concentrating in young-age children.