Abstract: Objective To explore the cognition of hepatitis C among medical staff and undergraduate medical students and to provide evidence for hepatitis C control.Methods With cluster sampling method,337 medical workers and 343 undergraduate medical students were selected for a questionnaire survey.Results The response rate was 93.25%.There were 96.44% of the medical staff and 90.38% of the medical students being aware of hepatitis C,with a significant difference between the two groups.The general awareness of medical staff about the transmission of hepatitis C was 69.57% and that of the students was 66.86%,with no significant difference between medical staff and medical students.Conclusion Efforts should be made to promote the level of the cognition on hepatitis C among medical staff and students.