Abstract: ObjectiveTo investigate the distribution of genetic polymorphisms of human glutathione.S transferase gene (GSTM1 and GSTT1) in Han nationality population of Hainan province. MethodsDNA fragments were amplified by multiplex polymerase chain reaction (multiplex PCR) and agarose gel electrophoresis was used to identify the genotypes of GSTM1 and GSTT1 gene.The data were analyzed with SPSS17.0 software. ResultsIn this study,the frequencies of GSTM1 null and GSTT1 null genotype were 56.5% and 40.8%,respectively,and the frequencies of the individuals with combination of GSTM1 null and GSTT1 null genotype were 25.2% in the population.There were no significant differences in the GSTM1 and GSTT1 genetic polymorphisms among the different groups of age or gender.There were no significant associations between the two genes in the normal population distribution and they were independent of each other.Genetic polymorphisms distribution was different in different ethnic,national and regional population. ConclusionBoth GSTM1 and GSTT1 genes exist polymorphisms in Han nationality population of Hainan province.