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刘荣, 杨巍巍, 姜元松, 王振宇. 松多酚对牛肺动脉内皮细胞抗氧化活性影响[J]. 中国公共卫生, 2014, 30(1): 80-82. DOI: 10.11847/zgggws2014-30-01-24
引用本文: 刘荣, 杨巍巍, 姜元松, 王振宇. 松多酚对牛肺动脉内皮细胞抗氧化活性影响[J]. 中国公共卫生, 2014, 30(1): 80-82. DOI: 10.11847/zgggws2014-30-01-24
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LIU Rong, YANG Wei-wei, JIANG Yuan-song.et al, . Antioxidant capacity of Pinus sylvestris L.polyphenlols in bovine pulmonary artery endothelial cells[J]. Chinese Journal of Public Health, 2014, 30(1): 80-82. DOI: 10.11847/zgggws2014-30-01-24
Citation: LIU Rong, YANG Wei-wei, JIANG Yuan-song.et al, . Antioxidant capacity of Pinus sylvestris L.polyphenlols in bovine pulmonary artery endothelial cells[J]. Chinese Journal of Public Health, 2014, 30(1): 80-82. DOI: 10.11847/zgggws2014-30-01-24
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松多酚对牛肺动脉内皮细胞抗氧化活性影响

Antioxidant capacity of Pinus sylvestris L.polyphenlols in bovine pulmonary artery endothelial cells

    摘要
  • 摘要: 目的 探讨松多酚对H2O2引起的牛肺动脉内皮细胞（BPAECs)氧化损伤的保护作用。方法 采用H2O2诱导BPAECs建立氧化损伤模型，实验设对照组、模型组、松多酚高、中、低（0.5、0.1、0.05 mg/mL)剂量组;噻唑蓝法检测细胞存活率，试剂盒法检测抗氧化酶活性、丙二醛含量等。结果 与对照组比较，模型组BPAECs存活率（0.71±0.03)%下降，超氧化物歧化酶（SOD)、过氧化氢酶（CAT)、谷胱甘肽过氧化物酶（GSH-Px)活力分别为（24.67±0.69)、（41.95±0.58)、（24.38±1.67）U/mg prot下降，MDA含量（5.83±0.04）mmol/mL升高;与模型组比较，松多酚0.5 mg/mL组BPAEJP2Cs可明显提高细胞存活率（1.64±0.03)%，SOD、CAT和GSH-PxJP酶活力分别为（53.62±1.24)、（61.01±1.10)、（48.62±0.82)U/mg prot明显升高，MDA含量（1.96±0.00)mmol/mL明显降低，蛋白质羰基含量降低;呈剂量-效应关系。结论 松多酚对H2O2诱导氧化损伤的BPAECs具有保护作用。

     

    Abstract: Objective To investigate protective effect of Pinus sylvestris L.polyphenlols extracts on H2O2 induced damage in bovine pulmonary artery endothelial cells (BPAECs).Methods Oxidative damage of BPAECs was induced by H2O2.The BPAECs were divided into control group,H2O2 model group,and high-,midium-,and low-dose polyphenlols groups (0.5,0.1,0.05 mg/mL).The influence of Pinus sylvestris L.polyphenlols extracts on H2O2 induced damage in BPAECs was assessed by measuring the cells' viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrozolium bromide(MTT)assay.The superoxide dismutase (SOD);malondialdehyde (MDA),and glutathione peroxidase (GSH-Px)content were determined with assay kits.Results The cell viability of BPAECs decreased (0.71±0.03%)in H2O2 model group compared to that of the control group.The kinases activity of SOD,catalase (CAT),and GSH-Px decreased (24.67±0.69,41.95±0.58,24.38±1.67 U/mg/prot).Meanwhile,the content of MDA increased (5.83±0.04 mmol/mL).In high-dose group (0.5mg/ml),the cell viability of BPAECs raised remarkably (1.64±0.03%)and the kinases activity of SOD,CAT,and GSH-Px improved obviously (53.62±1.24,61.01±1.10,and 48.62±0.82 U/mg/prot).The contents of MDA and protein carbonyl reduced markedly (1.96±0.00 mmol/mL,1.13±0.08 U/mg protein)compared to those of the H2O2 model group.The Results showed a dose-effect relationship.Conclusion The Pinus sylvestris L.polyphenlols extracts possess protective effect on H2O2 induced damage in EPAECs

     

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