Abstract: ObjectiveTo study the prevalence and viral etiology of acute upper respiratory infection among children in Ji'nan city,Shandong province and to provide reference for making preventive strategy,clinical diagnosis and treatment.MethodsFrom September 2010 to August 2011,we collected the samples of throat swab from the acute upper respiratory infection patients in Qilu Children Hospital of Shandong University.The samples were detected with real-time PCR for human bocavirus(HBoV)and multiplex PCR for 12 kinds of respiratory virus,respectively.Madin-Dardy canine kidney(MDCK)cells and Hep-2 cells were used to isolate influenza virus(FLU),human adenovirus(HAdV)and respiratory syncytial virus(RSV).ResultsTotally 84 positive samples were detected from the 174 samples,with a positive rate of 48.28%.HAdV(20.43%)was the most common virus,followed by Flu A(18.28%),human parainfluenza viruses -3(HPIV-3)(15.05%),RSVA(11.83%),human coronavirus 229E/NL63(HCoV-229E/NL63)(10.75%),Flu B(6.45%),human coronavirus OC43/HKU1(HCoV-OC43/HKU1)(5.38%),and RSVB(4.30%).For the co-infections,HAdV(22.22%)and RSVA(22.22%.2.)were strains mostly detected,followed by Flu A(16.67%).The children under 5 years were more likely to have Flu A and RSVA infection and persons ≥5 years old were more likely to have 12 HAdV influenza viruses and 9 HAdV strains.ConclusionThis study underlines the important of viral pathogens in acute upper respiratory infection in Ji'nan city,Shandong province.The major viral pathogens are HAdV,Flu A,HPIV-3,RSVA,HCoV-229E/NL63 and Flu B with different seasonal and special distributions.