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1,25二羟维生素D3对肝癌HepG2细胞增殖侵袭影响

娄方方 孙建超 黄健 孔维莹 黄音员祝

娄方方, 孙建超, 黄健, 孔维莹, 黄音员祝. 1,25二羟维生素D3对肝癌HepG2细胞增殖侵袭影响[J]. 中国公共卫生, 2016, 32(1): 81-84. doi: 10.11847/zgggws2016-32-01-24
引用本文: 娄方方, 孙建超, 黄健, 孔维莹, 黄音员祝. 1,25二羟维生素D3对肝癌HepG2细胞增殖侵袭影响[J]. 中国公共卫生, 2016, 32(1): 81-84. doi: 10.11847/zgggws2016-32-01-24
LOU Fang-fang, SUN Jian-chao, HUANG Jian.et al, . Effects of 1,25(OH)2D3 and its combination with LY294002 on proliferation and invasion of human hepatocellular carcinoma HepG2 cells[J]. Chinese Journal of Public Health, 2016, 32(1): 81-84. doi: 10.11847/zgggws2016-32-01-24
Citation: LOU Fang-fang, SUN Jian-chao, HUANG Jian.et al, . Effects of 1,25(OH)2D3 and its combination with LY294002 on proliferation and invasion of human hepatocellular carcinoma HepG2 cells[J]. Chinese Journal of Public Health, 2016, 32(1): 81-84. doi: 10.11847/zgggws2016-32-01-24

1,25二羟维生素D3对肝癌HepG2细胞增殖侵袭影响

doi: 10.11847/zgggws2016-32-01-24
基金项目: 

贵州省科技厅基金(黔科合LH字[2014]7114)

详细信息
    作者简介:

    娄方方(1988-),女,贵州遵义人,硕士在读,研究方向:临床生物化学。

    通讯作者:

    黄音员祝E-mail:374185146@qq.com

  • 中图分类号: R395.6

Effects of 1,25(OH)2D3 and its combination with LY294002 on proliferation and invasion of human hepatocellular carcinoma HepG2 cells

  • 摘要: 目的 探讨1,25二羟维生素D3[1,25(OH)2D3]及PI3K/AKT信号通路抑制剂(LY294002)对人肝癌HepG2细胞的增殖、侵袭影响及作用机制。方法 实验设10-8、10-7、10-6 mol/L 1,25(OH)2D3组及2.5、5.0、10.0、20.0、40.0、80.0μmol/L LY294002组,10-7 mol/L 1,25(OH)2D3+5μmol/L LY294002联合组,噻唑蓝法检测人肝癌HepG2细胞增殖抑制率;CompuSyn软件计算联合指数;Tanswell小室检测HepG2细胞侵袭数;Western blot检测HepG2细胞增殖细胞核抗原(PCNA),细胞基质金属蛋白酶9(MMP-9)、磷酸化丝氨酸苏氨酸蛋白激酶(p-AKT)、10号染色体缺失且与张力蛋白同源物磷酸脂酶基因(PTEN)蛋白。结果 1,25(OH)2D3及LY294002对人肝癌HepG2细胞增殖抑制率呈时间-剂量依赖效应(P<0.05);1,25(OH)2D3联合LY294002组细胞增殖抑制率明显高于二者单独处理组(P<0.05),联合指数=0.728,2者具有协同效应;10-7 mol/L 1,25(OH)2D3组、5μmol/L LY294002组以及联合组HepG2细胞侵袭数[分别为(45.9±6.4)、(49.9±6.0)、(27.8±4.0)个]明显低于对照组[(64.6±8.0)个](P<0.05)。与对照组比较,10-7 mol/L 1,25(OH)2D3组及联合组HepG2细胞PTEN蛋白表达量增加,差异有统计学意义(P<0.05),10-7 mol/L 1,25(OH)2D3组、5μmol/L LY294002组及联合组HepG2细胞PCNA、MMP-9、p-AKT蛋白表达均降低,差异有统计学意义(P<0.05),且联合组蛋白表达量低于二者单独处理组(P<0.05)。结论 1,25(OH)2D3可抑制人肝癌HepG2细胞增殖、侵袭,其机制可能与上调PTEN表达,抑制PI3K/AKT信号通路活性,下调PCNA、MMP-9蛋白有关;与LY294002合用具有协同效应。
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出版历程
  • 收稿日期:  2015-09-16
  • 刊出日期:  2016-01-10

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