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袁成福, 李志红, 彭帆, 肖方祥. 火棘多糖对CCl4所致大鼠肝损伤保护作用[J]. 中国公共卫生, 2016, 32(11): 1485-1487. DOI: 10.11847/zgggws2016-32-11-09
引用本文: 袁成福, 李志红, 彭帆, 肖方祥. 火棘多糖对CCl4所致大鼠肝损伤保护作用[J]. 中国公共卫生, 2016, 32(11): 1485-1487. DOI: 10.11847/zgggws2016-32-11-09
YUAN Cheng-fu, LI Zhi-hong, PENG Fan.et al, . Hepatoprotective effect of Pyracantha fortuneana polysaccharides on liver injury induced by CCI4 in rats[J]. Chinese Journal of Public Health, 2016, 32(11): 1485-1487. DOI: 10.11847/zgggws2016-32-11-09
Citation: YUAN Cheng-fu, LI Zhi-hong, PENG Fan.et al, . Hepatoprotective effect of Pyracantha fortuneana polysaccharides on liver injury induced by CCI4 in rats[J]. Chinese Journal of Public Health, 2016, 32(11): 1485-1487. DOI: 10.11847/zgggws2016-32-11-09

火棘多糖对CCl4所致大鼠肝损伤保护作用

Hepatoprotective effect of Pyracantha fortuneana polysaccharides on liver injury induced by CCI4 in rats

  • 摘要: 目的 探讨火棘多糖对四氯化碳(CCI4)所致大鼠肝损伤的保护作用。方法 将50只Wistar大鼠随机分为5组:对照组,CCI4模型组,联苯双酯(BP)组,火棘多糖200、400 mg/kg组。对照组大鼠中腹膜下注射橄榄油,其余各组大鼠腹膜下注射30% CCI4,联苯双酯组、火棘多糖组分别灌胃BP 200 mg/kg,火棘多糖200、400 mg/kg。5周后,测定血清中丙氨酸转氨酶(ALT)及天冬氨酸转氨酶(AST)含量;检测肝组织中丙二醛、谷胱甘肽过氧化物酶(GSH-Px)含量及总抗氧化能力(T-AOC)活性;用real time PCR检测大鼠肝组织中核因子相关因子2(Nrf2)及过氧化氢酶(CAT)mRNA表达变化。结果 与CCI4模型组大鼠血清ALT、AST含量(521.42±68.21)、(589.17±39.44)U/L比较,火棘多糖200、400 mg/kg组大鼠血清ALT、AST含量分别为(221.13±28.46)、(190.31 ±27.32)与(286.4±31.57)、(224.6±30.52)U/L明显降低,差异有统计学意义(P<0.05);与CCI4模型组大鼠肝组织中丙二醛含量(32.14±3.26)nmol/mg pro比较,火棘多糖200、400 mg/kg组大鼠肝组织中丙二醛含量分别为(23.29±4.38)、(18.67±3.28)nmol/mg pro明显降低,差异有统计学意义(P<0.05);与CCI4模型组大鼠肝组织中T-AOC活性(73.15±9.23)U/mL比较,火棘多糖200、400 mg/kg组大鼠肝组织中T-AOC活性分别为(106.32±14.23)、(152.31±16.12)U/mL明显升高,差异有统计学意义(P<0.05);real time PCR结果显示,与CCI4模型组比较,火棘多糖组大鼠肝组织中Nrf2及CAT mRNA表达水平增加。结论 火棘多糖对CCI4所致大鼠肝损伤具有保护作用,其机制可能与火棘多糖可提高大鼠抗氧化能力有关。

     

    Abstract: Objective To study hepatoprotective effect of Pyracantha fortuneana polysaccharides on liver injury induced by carbon tetrachloride (CCI4) in rats.Methods Fifty Wistar rats were randomly divided into five groups:control group,CCI4 group,biphenyldicarboxylate pills (BP) group,and 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups.The rats of control group were given olive oil by intraperitoneal injection (IP) and those of other groups were given 30% CCI4 by IP,respectively.The rats of BP group were given BP (200 mg/kg) by gavage and the rats of Pyracantha fortuneana polysaccharides groups were given Pyracantha fortuneana polysaccharides of 200 and 400 mg/kg by gavage,respectively.Five weeks after the treatments,the contents of serum alanine transferase (ALT) and aspartate transaminase (AST) were determined; the levels of malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) and the activity of total antioxidative capacity (T-AOC) in liver were measured.The mRNA expression levels of nuclear factor erythroid 2-related factor (Nrf2) and catalase (CAT) were detected with real time PCR.Results The serum ALT (221.13±28.46 and 190.31 ±27.32 U/L)and AST (286.4±31.57 and 224.6±30.52 U/L)decreased remarkably in the rats of 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups compared to those of the rats in CCI4 group (521.42±68.21 and 589.17±39.44 U/L) (all P<0.05).The MDA (23.29±4.38 and 18.67±3.28 nmol/mg pro) in liver of the rats of 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups decreased remarkably compared to that of rats in the CCI4 group (both P<0.05).The T-AOC (106.32±14.23 and 152.31±16.12 U/mL) in liver of the rats in 200 and 400 mg/kg Pyracantha fortuneana polysaccharides groups increased significantly compared to that (73.15±9.23 U/mL)of the rats in CCI4 group (both P<0.05).The mRNA expression levels of Nrf2 and CAT increased in the liver of rats in Pyracantha fortuneana polysaccharides groups compared with those of the rats in CCI4 group.Conclusion Pyracantha fortuneana polysaccharides administration is effective in attenuating CCI4-induced liver injury and the effects may be related to the enhancement of antioxidant activity of Pyracantha fortuneana polysaccharides in rats.

     

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