宜兴地区丙型肝炎病毒基因型及变异情况分析
Analysis on hepatitis C virus genotype and quasispecies in Yixing area
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摘要:目的 了解江苏宜兴地区丙型肝炎病毒(HCV)基因型分布特点和变异情况对疾病的影响。方法 对宜兴市人民医院收治的166例HCV抗体阳性患者血清进行HCV RNA检测, 采用5'非编码区(5'NCR)1, 2, 3, 1 b型特异性引物, 对阳性标本进行Simmonds分型。选取未经干扰素治疗的43份慢性丙型肝炎患者的HCV RNA阳性血清, 采用RT-PCR扩增HCV包膜区(E2), 单链构象多态性聚合酶链反应法(SSCP)获得HCV准种条带数, 准种数 > 2为复杂准种, 比较不同肝病活动度患者的准种复杂情况。结果 166份血清中, 有102份为HCV RNA阳性。其中1 b型86份占84.3%, 2型6份占5.9%, 1 b/2型的混合感染5份占4.9%, 不能分型的5份占4.9%。男性和女性在基因型总体分布差异有统计学意义。在单一型感染和混合型感染的分布差异亦有统计学意义, 而在不同年龄、不同临床类型的肝炎中分布差异无统计学意义。HCV包膜区准种复杂与否和丙氨酸转氨酶(ALT)的正常与否具有相关性。结论 江苏宜兴地区的HCV感染主要为1 b型, 病毒的变异程度与ALT成正相关。Abstract:Objective To investigate the distribution of hepatitis C virus(HCV)genotypes and HCV quasispecies diversity in Yixing, Jiangsu province.Methods To identify the genotype of sera samples obtained from 163 donors who anti-HCV positive by the PCR with type specific primer, which were designed according to the sequence of 5cono-coding region(5'NCR). Genotypes distribution was investigated in patients with different sex, different ages and different kinds hepatitis C. To compare the HCV quasispecies diversity among the 43 patients with chronic hepatitis C.Results Of total 166 patients, 102 were HCV RNA positive in which 86 patients had genotype 1b(86/102;84.3%), 5 patients had genotype 2(6/102;5.9%), 5 patients was 1b/2 mixed types(5/102;4.9%)and genotype could not be determined in 5 patients. Distribution difference of HCV genotypes was found between female patients and male ones. The difference of HCV quasispecies diversity was found between the patients with normal or abnormal alanine transaminase(ALT).Conclusion Type 1b is predominant HCV genotype in Yixing area. Mix-infection rate is higher in females than that in males. Quasispecies diversity is corrected with ALT.
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