日本血吸虫31/32kDa重组抗原细胞免疫机制

陈喜珪; 沈定文; 罗金萍; 覃金红; 彭胜国

日本血吸虫31/32kDa重组抗原细胞免疫机制

Study on protective cell immune mechanism of recombinant 31/32kDa antigens of Schistosoma japanicum

摘要

摘要:
目的探讨细胞免疫在重组日本血吸虫31/32kDa抗原(rSj31/32)保护性免疫机制中的作用。
方法采用rSj31/32抗原免疫BALB/c小鼠, ELISA分别检测免疫前后小鼠血清干扰素(IFN-γ)和白细胞介素(IL-4)的含量, 攻击感染后以脾细胞培养法检测经刀豆蛋白(ConA)和可溶性虫卵抗原(SEA)刺激后, 小鼠脾细胞分泌IFN-γ和IL-4的水平。
结果 rSj31/32抗原免疫后血清IFN-γ的水平明显增加(P < 0.001), 而IL-4的含量无明显变化; 经SEA刺激, 免疫组脾细胞产生较高水平的IFN-γ, 而产生的IL-4水平较其他组低(P < 0.01)。
结论细胞免疫在rSj31/32诱导的保护性免疫中起着重要作用。

Abstract:
Objective To study the effect of cell immunity in the protective immune mechanism of recombinant rSj31/32kDa antigens of Schistosoma japonicum(rSj31/32).
Methods BALB/c mice were immunized three times with rSj31/32. ELISA kits were used to examine the levels of IFN-C and IL-4 before and 6 weeks after immunization. By the culture of spleen cells after challenge, IFN-C and IL-4 after stimulation with SEA were quantified by ELISA kits.
Results The level of IFN-Cwere increased significantly a-f ter immunized with rSj31/32(P < 0.001). There was no obvious change in the level of IL-4. With spleen cells from rSj31/32 vaccinated mice, IFN-C was the most abundantly produced cytokine in response to SEA, while IL-4 secretion was the lowest(P < 0.01).
Conclusion Cell immunity might play an important role in the protective immunity elicited by rSj31/32 antigen.

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