SARS病例尸解标本和咽嗽液中病原体检测

晏辉钧; 赵卫; 方丹云; 周经姣; 张文炳; 龙北国; 郭辉玉; 江丽芳

SARS病例尸解标本和咽嗽液中病原体检测

Detection of etiologic agents of autopsy lung of dead cases and pharyngeal gargles of Severe Acute Respiratory Syndrome

摘要

摘要:
目的确定严重急性呼吸系统综合征(SARS)病例尸解肺组织和咽嗽液中病原体种类。
方法用超薄切片电镜技术观察广东省传染性非典型肺炎流行早期3例死亡病例尸解肺组织标本, 用RT-PCR法扩增尸解肺组织和SARS病人咽嗽液中SARS冠状病毒特异核酸片段, 并进行序列测定。
结果在死亡病人肺组织中观察到衣原体的网状体、中间体以及原体颗粒, 并见衣原体包涵体样结构; 用RT-PCR法在2例尸解肺组织扩增出预期大小的DNA片段, 测序后经相似性(BLAST)比较, 其与SARS冠状病毒相应基因片段高度同源; 用巢式RT-PCR技术在SARS病人咽漱液中扩增出SARS冠状病毒特异基因片段。
结论在SARS尸解肺组织中发现衣原体样颗粒, 在尸解肺组织和咽漱液中扩增出SARS冠状病毒核酸片段, 说明SARS患者可合并衣原体等微生物混合感染。

Abstract:
Objective To detect the etiologic agents of severe acute respiratory syndrome(SARS).
Methods The autopsy lung of the 3 dead cases of SARS were collected from Guangdong for thin-section electron microscopic examination.The gene fragments of SARS-CoV from the autopsy lung and pharyngeal gargles were amplified by reverse transcription-polymerase chain reaction(RT-PCR), and followed by sequence analysis.
Results Chlamy dia-like agent were visualized in all lung tissues of three cases with SARS.Specific gene fragments were amplified from the lung samples of two cases with SARS, which were comfired by DNA sequencing of Blast to belong to SARS-CoV.
Conclusion Chlamydia-like particles were found in EM, and SARS-CoV could be detected by RT-PCR from the lung samples and pharyngeal gargles with SARS.

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