HCV感染者和HCV/HIV合并感染者免疫调节

康辉; 尚红; 刁莹莹; 范霞; 代娣; 姜桐军; 耿文清

HCV感染者和HCV/HIV合并感染者免疫调节

Analysis on CD4~+CD25~+Tcell in HCV infection and HCV/HIV co-infection

摘要

摘要:
目的探讨我国单纯丙型肝炎病毒(HCV)感染者和HCV/HIV合并感染者免疫应答的相关机制。
方法分离人外周血单个核细胞; 流式细胞仪(FACS)检测CD4+T细胞和CD4⁺CD25⁺T细胞表达水平。
结果 CD4⁺CD25⁺T细胞占外周血单个核细胞中CD4⁺T细胞比例(%CD4⁺CD25⁺), HCV感染组明显高于健康对照组(19.2% > 138%, P < 0.05), HCV/HIV合并感染组明显低于对照组(6.9% < 13.8%, P < 0.001), 更明显低于HCV感染组(P < 0.001)。
结论 HCV感染者体内CD4⁺CD25⁺T细胞增殖明显, 提示CD4CD25⁺T细胞在HCV慢性感染中发挥免疫调节作用。HCV/HIV合并感染时, CD4⁺CD25⁺T细胞受损, 从而影响免疫调节功能。

Abstract:
Objective To investigate the characteristic of cellular immune responses in chronic HCV patients and HCV/HIV coinfection patients from China.
Methods Human peripheral blood monocytes were isolated.The frequency and percentage of CD4⁺ T lymphocytes and CD4⁺CD25⁺T lymphocytes were determined by FACS.
Results The higher proportion of CD4⁺ CD25⁺ T lymphocytes was found in chronic HCV infection than that of normal control(19.2% > 13.8%, P < 0.105), while significant lower percentage was found in HCV/HIV coinfection than normal control(6.9% < 13.8%, P < 0.001)or chronic HCV infection(P < 0.001).
Conclusion The individuals have a strong CD4⁺ CD25⁺ T cells proliferation against HCV infection while a week CD4⁺ CD25⁺ T cells against HCV/HIV co-infection.It suggests that CD4⁺ CD25⁺ T lymphocytes plays an immune regulatory role in HCV persistence.HCV/HIV co-infection would impair the CD4⁺ CD25⁺ T lymphocytes, the immune regulatory function would be affected.

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