胃癌患者癌组织中EB病毒感染相关性分析

章广玲; 周天戟; 刘志勇; 张雪怡; 李淑英

胃癌患者癌组织中EB病毒感染相关性分析

Analysis on Epstein-Barr virus infection in tissues of gastric carcinoma in Tangshan

摘要

摘要:
目的调查唐山地区胃癌组织中EB病毒的感染状况, 以分析EB病毒相关胃癌(GC)的临床病理学特征, 以及EB病毒在胃癌形成过程中的作用。
方法取自唐山市工人医院(130例)、开滦医院(57例)和唐山市卫生学校附属医院(15例)病理科2001~2003年储存的胃癌患者手术切除后石蜡包埋的组织标本共202例。标本经切片和常规脱蜡处理后提取DNA, 经PCR进行βactinDNA检测, 阳性标本用PCR法检测EB病毒核抗原1(EBNA1)DNA。
结果 202份标本βactinDNA阳性166例, 阳性率为82%;166例标本中EBNA1PCR阳性14例, 阳性率为8.4%;166例βactinDNA阳性的胃癌标本中, 腺癌150例, 非腺癌16例, 残胃癌15例。经EBNA1PCR检测EB病毒DNA阳性例数在腺癌、非腺癌和残胃癌中分别为14, 0, 5, 阳性率分别为9.3%, 0%。33.3%。
结论 EB病毒感染在唐山地区胃癌形成过程中可能起到一定的作用。

Abstract:
Objective To determine the occurring frequency, and to clarify the clinic pathological characteristics, and to analyze the roles of EBV in the diffusion and metastasis of EBV-related gastric carcinoma in Tangshan area.The situation of EBV infection was investigated in the gastric carcinoma with different histological types in Tangshan Ares.
Methods 202 cases of formalin-fixed and paraffin-embeded tissue came from surgically resedted gastric carcinomas in Tangshan area, were confirmed further by histopathological observation by hematoxylin-eosin(HE)staining.The DNAs were extracted according to the method described by Van Beek with some modifications, and were screened for B-actin DNA detection.EBV infection was analyzed by detecting EBNA1 DNA by PCR.
Results EBVDNA were detected in 14 in 166 B-actin DNA positive cases (81.4%).14 positive cases were come from 150 adenocarcinomas(91.3%), and 5 from 15 gastric stumps(33.3%), and 0 from 16 non-adenocar cinomas(0%).
Conclusion The occurring rate of EBV-related gastric carcinoma is 8.4% in Tangshan area, which suggests that EBV infection is likely to be involved in the occurrence or development of gastric carcinoma.The infectious rate is significantly higher in gastric stumps than that in other gastric carcinomas.

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