HIV-1感染相关基因突变频率及多态性研究
Study on polymorphism of chemokine receptor alleles influencing genetic susceptibility to HIV-1 infectionin
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摘要:目的 研究山东省济南市人群中与人类免疫缺陷病毒(HIV)-1感染相关的CCR2-64I和SDF13'A等位基因的突变频率和多态性特点。方法 以234例济南市普通人群为研究对象, 来自商河和章丘2个县, 其中无1例是HIV感染者, 从上述人群外周血的外周血单干核细胞(PBMC)中抽提基因组DNA, 然后应用PCR/限制性片断长度多态性(RFLP)检测去化因子受体(CCR)2-64I和基质细胞衍生因子(SDF)1-3多态性。结果 济南市人群中存在CCR2-64I和SDF1-3'A等位基因突变, 2种突变频率分别为20.25%和21.00%, 两等位基因型的分布均符合Hardy-Weinberg平衡。结论 济南市人群CCR2-6.4I和SDF1-3'A等位基因的突变频率较高, 人群对HIV-1感染的遗传易感性相对较低。Abstract:Objective Mutant frequency and polymorphism of HIV-1 resistance CCR-61I and SDF1-3' Aalleles were investigated in the population from Jinan Shandong province.Methods The study population included 234 subjects from Jinan city.They came from Shanghe and Zhangqiu county; and none was HIV-1 positive.The genotypes of the two mutations were respectively detected by PCR/RFLP(restriction fragment length polymorphism)assay with the digestion of restriction endonuclease Bsa BI and MspI for CCR2-64I and SDF1-3' A mutation.Results The frequency of the mutant alleles were: 20.25% for CCR2-64I and 21.00% for SDF1-3' in Jinan city, Shandong province.Conclusion There were high frequencies of CCR2-64I and SDF1-3' Aalleles in Jinan population.The genetic susceptibility to HIV-1 infection and AIDS disease progression was low in Jinan city.
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