绿茶提取物的安全性分析评价

Safety assessment of extract from green tea

摘要:
目的对调节血糖产品-绿茶提取物的安全性进行评价。
方法采用急性经口毒性试验、蓄积毒性试验、小鼠骨髓嗜多染红细胞微核试验、V₇₉细胞基因突变试验对绿茶提取物的急性毒性、蓄积毒性、遗传毒性进行观察。
结果该提取物对雌、雄性小鼠急性经口LD₅₀均 > 1000 0mg/(kg·bw), 属于实际无毒级; 对动物蓄积系数K > 5, 属于弱蓄积性; 对小鼠骨髓细胞未产生致畸变作用; 对体外培养的中国仓鼠肺细胞(V₇₉), 无论直接作用和代谢活化后作用, 均未呈现致突变性。
结论绿茶提取物是一种安全的调节血糖制剂。

Abstract:
Objective To test the toxicity of an extract from green tea used for blood glucose regulation.
Methods Acute oral toxicity test, cumulative toxicity test, in vivo mammalian bone marrow cytogenetics test and in vitro mammalian cell gene mutation test were applied on it.
Results The median lethal dose(LD₅₀)for female and male mice was more than 10 000 mg/kg body weight.It belonged to actual non-toxic substance.The extract had weak accumulation in mice(accumalation index > 5), and it did not show distortion effect on mice marrow cell.It did not have mutative effect on V₇₉ cell either directly or activated.
Conclusion The extract from green tea is a safty agent for diabetes mellitus patients.