Abstract:
Objective To analyze micronuclei of mice peripheral blood reticulocytes induced by cadmium chloride(CdCl2) with flow cytometry(FCM)and to explore the genotoxicity of CdCl2 and further to evaluate the application value of FCM in micronuclei assay.
Methods Mice were randomly divided into 10 groups(n=5)and exposed to 10-60mg/kg cyclophosphamide(CP)and 50-400μg/kg CdCl2 respectively.The micronucleated reticulocytes(MN-RET)in per ipheral blood of mice were detected by a flow cytometry-based method, in which anti-CD71-FITC and propidiumiodide(PI)were used to stain cells and malaria-infected erythrocytes were used as a biological standard of micronuclei to optimize cytometry.
Results The frequency of MN-RET in peripheral blood(auto matically detected with FCM)and bone marrow(assayed with microscopy)was significantly increased with the highly correlation between them(r=0.955).No remarkable alteration in micronuclei of peripheral reticulocytes was observed in mice exposed to different concentration of CdCl2.
Conclusion The frequency of MN-RET in peripheral blood with FCM could be used to assess the micronucleus formation effect of genotoxicity.No significant micronucleus formation was found in cadmium-poisoned mice.