氨基末端激酶信号通路在细跑凋亡中的作用
Effects of c-Jun N-terminal kinase signal pathway on cell apoptosis
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摘要:目的 观察维生素E琥珀酸酯(VES)和维生素E(VE)对人B细胞白血病细胞株(Raji)的诱导凋亡作用及c-Jun氨基末端激酶(JNK)信号通路在VES诱导细胞凋亡中的作用。方法 应用流式细胞仪、免疫印迹法(Westernblot)等技术方法, 体外观察VES和VE对Raji细胞的作用。结果 VES和VE对Raji细胞均有程度不一的诱导凋亡作用, VES处理后6h, 在DNA直方图上G1峰前出现一个明显的凋亡峰, 并随着VES作用时间延长而增强, 具有时间效应关系, 而VE诱导凋亡的作用则相对较弱, 同时VES作用后, 细胞内磷酸化型JNK蛋白于6h开始增高, 12h达到高峰, 并持续24h, 而非磷酸化型JNK蛋白表达无显著变化。结论 VES是JNK的激活因子, JNK信号通路的激活在VES诱导肿瘤细胞凋亡的过程中发挥重要作用。Abstract:Objective To investigate the effects of vitamin Esuccinate(VES) and vitamin E(VE)on cell apoptosis and the role of c-Jun N-terminal kinase in vitamin E succinate-induced cell apoptosis in Raji cells.Methods Flow-cytometry and Western Blotting analysis were used to observe the effects of VES and VE on Raji cells in vitro.Results Both VES and VE could induce apoptosis in Raji cells.VES was more effective than VE, which induced apoptosis in time-dependent manner.In Raji cells treated with VES, the level of phosphorylated form of JNK increased at 6 h and reached peak level at 12 h, which continuing through 24 h after treatment initiation.The level of phosphorylation state-independent JNK was not changed.Conclusion VES was a potent activator of JNK, which played an important role in the apoptosis induced by VES in human cancer cells.
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