对硝基苯酚与杂交中DNA的相互作用

Study on interaction between nitrophenols and DNA in hybridization

摘要:
目的探讨对硝基苯酚与DNA的相互作用方式。
方法运用循环伏安法、荧光光谱法、紫外可见光谱法、DNA分子杂交技术、量子化学方法, 研究pH为7.42磷酸盐缓冲溶液中对硝基苯酚与DNA的相互作用。
结果 DNA在杂交过程中与对硝基苯酚形成DNA加合物, 对硝基苯酚的荧光光谱曲线、紫外光谱曲线的吸收峰改变较大, 氧化峰电流下降, 量化计算表明对硝基苯酚位于DNA的碱基附近。
结论在DNA杂交过程中对硝基苯酚与DNA碱基可能以Π-Π方式结合。

Abstract:
Objective To study the interaction between nitrophenols and deoxgribonucleic acid in hybridization.
Methods Ultraviolet-visble spectrophotometry, fluorescence spectrophotometry, cyclic voltammetry, hybridization and quantum chemistry were used to study the interaction between nitrophenols and deoxgribonucleic acid.
Results In pH 7.42 sodium phosphate buffer solution, the complex could be formed in hybridization, which decreased the deoxidized current of nitrophonals, and changed the peaks of absorption and decreased the deoxidized current of nitrophonals, and changed the peaks of absorption and fluorescence spectra.The theoretical calculation indicated that the nitrophenols closed with the base groups of DNA.
Conclusion Ⅱ-Ⅱ bonding was the major modes for the interaction between nitrophenols and DNA in hybridization.