急慢性砷染毒的肝细胞L-02的基因芯片分析

杨磊; 顾永清; 潘泽民; 袁红琳; 仙玲玲; 王国荃; 应康; 李遥; 谢毅

急慢性砷染毒的肝细胞L-02的基因芯片分析

Analysis of gene chip on acute arsenite-induced and chronic arsenite-induced L-02 Cells

摘要

摘要:
目的用基因芯片分析急慢性砷染毒时人正常肝细胞(L-02细胞)基因表达谱的变化。
方法 6μmol/L的亚砷酸钠染毒L-02细胞2, 15, 24h, 4μmol/L的亚酸钠染毒L-02细胞2周, 分别与未染毒的L-02细胞抽提RNA作表达谱基因芯片杂交。
结果急性和慢性砷诱导细胞基因表达谱截然不同.染砷后首先激活的是解毒功能相关的基因和一些蛋白质合成相关的基因.长期染砷后与肿瘤发生及氧化还原有关的基因表达量升高。
结论细胞短期染砷毒后, 应激和解毒功能有关的蛋白表达上调, 长期染砷后与肿瘤有关的基因表达上调, 急慢性砷刺激下细胞以不同的方式抵抗砷毒。

Abstract:
Objective cDNA microarrays were used to investigate the expression profile of human genes in response to acute and chronic sodium arsenite treatment.
Methods The RNA was purified from the L-02 cells without and with arsenite sodium induct ion for 2, 15, 24h and 2 weeks respectively.
Results The hybridization patterns were quite different between acute and chronic sodium arsenite treatment.The genes related with detoxification function and protein synthesis function were activated at the acute arsenite induct ion.However after long-term exposure to arsenite, tumor-related genes were expressed highly, while immunity-related genes were inhibited.
Conclusion The genes related with detoxification function were activated at the acute arsenite induction.However, under long-term arsenite exposure, the differentially expressed genes could give a clue to carcinogenesis of arsenite.The ways of resistance to arsenite toxicity were different between acute and chronic sodium arsenite treatment.

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