番茄汁对人前列腺癌细胞DNA损伤作用的影响

Effect of tomato juice on DNA damage of human prostate carcinoma cell

摘要:
目的探讨番茄汁对人前列腺癌细胞株(PC-3)DNA损伤作用的影响。
方法于人前列腺癌PC-3细胞的细胞培养液中分别加入番茄汁40, 80, 120μl/ml, 培养48h;用单细胞凝胶电泳(SCGE)检测番茄汁对人前列腺癌PC-3细胞DNA损伤程度。
结果番茄汁可引起PC-3细胞DNA链断裂, 细胞尾长与拖尾率显著高于对照组, 差异有统计学意义(P < 0.01)。
结论番茄汁能导致人前列腺癌PC-3细胞的DNA损伤。

Abstract:
Objective To study the effect of tomato juice on the growth of human prostate carcinoma cell (PC-3).
Methods PC-3 cell was treated by the tomato juice with 40, 80, 120 l/ml for 48 h; Single cell gel electro phoresis(SCGE) assay was applied to determine the DNA damage of PC-3 cell.
Results Tomato juice could induce the breakage of DNA strand of PC-3 cell; Rate of DNA tail and the tail length of experimental groups were significantly higher than that of control group, with very statistical significance.
Conclusion Tomato juice could lead to DNA damage of PC-3 cell.