肿瘤特异性凋亡基因对人淋巴瘤细胞诱导作用

Apoptosis induced by Apoptin Gene in human lymphoma U937 Cells

摘要:
目的观察肿瘤特异性凋亡基因(Apoptingene)对人淋巴瘤细胞U937的凋亡诱导作用。
方法用含有肿瘤特异性凋亡(Apoptin)基因的真核表达载体瞬间转染体外培养的人淋巴瘤细胞U937;采用台盼蓝染色法、RTPCR及DNA凝胶电泳等方法研究其在不同时间条件下对人淋巴瘤细胞U937诱导凋亡的作用。
结果 Apopin基因瞬间转染的人淋巴瘤细胞U937可出现明显的细胞凋亡; 而且凋亡细胞的数量与Apoptin的转染时间有关。
结论 Apoptin基因具有诱导人淋巴瘤细胞U937凋亡的作用。

Abstract:
Objective To explore apoptosis induced by apoptin gene in human lymphoma cell U937.
Methods The human lymphoma cell U937 was transient transformed by pcDNAA3 plasmids which contained apoptin gene, and apoptosis were measured by DNA agarose electrophoresis, RT-PCR and ELSIA at various time period.
Results The apoptin gene could induce apoptosis of human lymphoma cell U937 in vitro at 48 h after transformation.The mRNA of apoptin gene couldbe detected by RT-PCR at 24 h after transformation.Survial cells were associated with time after transformation.
Conclusion Apoptin gene can induce apoptosis in human lymphoma cell U937.