甲醛致V79细胞DNA交联作用的体外研究
Study on DNA crosslinks in V79 Chinese hamster cells exposed to formaldehyde in vitro
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摘要:目的 研究甲醛对V79细胞DNA的交联作用, 探讨甲醛的基因毒作用及可能的形成机制。方法 中国仓鼠肺细胞(V79)分别暴露于浓度为75, 150, 300, 600, 1200μmol/L的甲醛溶液中1, 2, 4h, 以紫外线照射作为标准断裂剂, 用单细胞凝胶电泳技术对不同浓度、不同暴露时间甲醛诱导的V79细胞DNA-交联作用进行体外实验。结果 在本研究所用的剂量范围内, 除75μmol/L组外, 其它4个浓度组3个染毒时间点甲醛均可引起显著的V79细胞DNA的交联作用, 彗星尾长和彗星细胞率显著低于紫外线照射的对照组(P < 0.01), 随着甲醛浓度的增加, 交联作用加强, 彗星尾长存在明显剂量效应关系(P < 0.01).且细胞暴露在甲醛中4, 2h;150, 300, 600μmol/L浓度组彗星尾长和彗星细胞率显著低于暴露于1h的相应浓度组(P < 0.01)。结论 甲醛是一种DNA交联剂, 具有诱导V79细胞DNA交联的作用, 并有明显的剂量、时间效应关系。Abstract:Objective To explore genotoxic effects and possible mechanism of formaldehy de on the DNA crosslinks.Methods V79 Chinese hamster cells were exposed to for maldehy de(FA)at different concentration of 75, 150, 300, 600, 1 200μmol/L and incubated for 1, 2, 4h, respectively.UV were used as standard DNA breaking agents, the DNA crosslinks in V79 Chinese hamster cells induced by different concentration of formaldehyde were detected with Single cell gel electrophoresis assay(SCGE, comet assay)in vitro.Results Formaldehyde could cause DNA crosslinks of V79 Chinese hamster cells within the given doses.Except for the 75μmol/L dose, the crosslinks were increased with the concentration of formaldehyde and the exposed time in vitro.Conclusion Formaldehyde was a DNA crosslinks agent that could cause DNA cross-links in V79 Chinese hamster cells, and the relationship between DNA crosslinks and concentration was a dose and time-dependent manner in vitro assay.
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