白念珠菌二相性与致病性关系

苑天红; 王明永; 吴升伟; 王正蓉; 吴承龙

白念珠菌二相性与致病性关系

Relationship between dimorphism and pathogenicity in candida albicans

摘要

摘要:
目的探讨白念珠菌二相性与致病性的关系。
方法分别将孢子相、菌丝相白念珠菌与人口腔颊粘膜细胞(BEC)进行粘附试验, 比较二相性白念珠菌对BEC的粘附率; 用兔抗人sIgA血清和人唾液sIgA, ¹²⁵I-sIgA放射免疫分析法检测唾液中sIgA的浓度, 比较含sIgA及不含sIgA的唾液对二相性白念珠菌粘附BEC的影响; 分别用小鼠腹腔中性粒细胞(PMN)、巨噬细胞(MΦ)对二相性白念珠菌进行吞噬实验, 研究对二相性白念珠菌的吞噬作用。
结果菌丝相白念珠菌对BEC的粘附率显著高于孢子相(P < 0.001);含sIgA的唾液抑制孢子相及菌丝相白念珠菌粘附BEC的能力强于不含sIgA的唾液(P < 0.005), 且唾液中sIgA抑制孢子相白念珠菌粘附BEC的能力强于抑制菌丝相白念球菌粘附BEC的能力(P < 0.05);PMN、MΦ对孢子相白念珠菌的吞噬作用强于菌丝相白念珠菌(P < 0.01)。
结论菌丝相白念珠菌的致病力强于孢子相白念珠菌的致病力。

Abstract:
Objective To study the relation between dimorphism and pathogenicity in candida albicans.
Methods Yeast forms and hyphal forms of candida albicans were used respectively to bind to BEC in vitro and the adherence rate of hyphal forms was compared with that of yeast forms.Rabbit anti-human serum was used to neutralize salivary sIgAand ¹²⁵I-sIgA radio immuno-assay was used to check the consistency of salivary sIgA for researching the effect of salivary sIgA on the adhesion of dimorphic candida albicans.Murine peritoneal polymorphonudeated neutrophils(PMN)and macrophages(MΦ)were used to engulf dimorphic candida albicans for researching the englobe of phagocytes to dimophic candida albicans.
Results The adherence of hyphal forms of candida albicans to BEC was stronger than that of yeast forms(P < 0.001).Salivary sIgA could inhibit the adherence of two forms ofcandida albicans to BEC(P < 0.005)and it was much easier to inhibit the adherence of yearst forms ofcandida albicans to BEC(P < 0.05).The ability of PMN, MΦ englobing yeast forms ofcandida albicans was more stronger than that of PMN, MΦ englobing hyphal forms of candida albicans(P < 0.01).
Conclusion The pathogenicity of hyphal forms was greater than that of yeast forms in candida albicans.

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