桑黄提取物对肿瘤生长和细胞免疫功能的影响
Effect of extract from phellinus igniarius on tumor growth and cell immunity function
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摘要:目的 探讨桑黄提取物对荷瘤小鼠肿瘤生长和细胞免疫功能的影响及抗肿瘤作用最佳剂量的筛选。方法 将清洁级C57小鼠按体重随机分成5组, 分别为空白对照组、阴性对照组、高剂量组、中剂量组和低剂量组.建立肝癌H22皮下移植瘤模型, 在普通饲料饲喂的基础上采用灌胃法, 空白对照组和阴性对照组给以生理盐水, 高、中、低剂量组分别给以不同剂量桑黄提取物, 观察其对肿瘤生长的影响; 利用小鼠腹腔巨噬细胞吞噬鸡红细胞试验检测巨噬细胞功能, 乳酸脱氢酶法检测自然杀伤细胞(natural killer cell, NK)活性, 噻唑蓝(MTT)法检测LAK细胞(Lymphokine activated killer cell)活性等细胞免疫功能的变化, 并观察其对脑、胸腺、肝、脾组织脏器指数的影响。结果 桑黄提取物干预组与对照组相比, 在瘤体比、抑瘤率及细胞免疫功能方面均具差异有统计学意义, 对主要脏器的影响差异无统计学意义, 其抗肿瘤作用的最佳剂量为200mg/(kg°bw)。结论 桑黄提取物对肿瘤生长具有抑制作用, 并可显著增强荷瘤小鼠的细胞免疫功能, 其抗肿瘤作用的最佳剂量为成人日常推荐摄入量3g/d的4倍。Abstract:Objective To explore effect of extract from phellinus igniarius on tumor growth and function of cell mediated immunity in mouse models.Methods C57 mice were divided into 5 groups by body weight randomly; they were the blank control, negative control and 3 treat groups.Subcutaneous transplantation H22 tumor mouse models were established.Besides of normal feeding, the control mice were fed with physiological saline and the treated mice were fed with different dose of phellinus igniarius to evaluate the tumor response; By experiment of phagocyte in abdominal cavity to phagocytize chicken red blood cell in vitro, method of lactic dehyrogenase and MTT, activity of mono phagocyte NK cell and LAK cell was checked respectively.The treatment effects on index of viscera of brain, thymus gland, liver and spleen were observed.Results Differences between treated groups and control groups in the rate of inhibition and function of cell mediated immunity were statistical significance, but visceral indexes was not statistical significance.The effects of treatment on 4 major visceras was not statistical significance.The optimal feeding dose was 200 mg/(kg°bw).Conclusion Extract from phellinus igniarius could inhibit tumor growth; enhanced function of cell mediated immunity of tumor-bearing mice and the optimal feeding dose was 4 times of the recommendatory dose for human being.
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