2, 5己二酮对大鼠大脑组织骨架蛋白的影响

张天亮; 赵秀兰; 谢克勤; 朱振平

2, 5己二酮对大鼠大脑组织骨架蛋白的影响

Effects of 2, 5-hexanedione on cytoskeletal proteins in cerebrum of rats

摘要

摘要:
目的探讨2, 5己二酮(HD)对大脑组织中神经丝、微管、微丝等骨架蛋白的影响。
方法 Wistar雄性大鼠, 经腹腔染毒, 剂量分别为200和400mg/(kg.d), 连续8周(每周5次), 采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western印迹方法检测大脑组织匀浆28000g、30min离心后所得上清和沉淀中低分子量神经丝(NF-L)、中分子量神经丝(NF-M)、高分子量神经丝(NF-H)、β-肌动蛋白、α-管蛋白和β-管蛋白的相对含量。
结果上清中NF-L和NF-M未能检测到, 其它神经丝亚单位的含量在沉淀和上清中均明显降低, 下降至对照组的33%~73%, 且呈现良好的剂量-反应关系.β-肌动蛋白、α-管蛋白、β-管蛋白的含量与对照组相比无明显变化。
结论 HD导致大鼠大脑中神经丝含量的降低, 神经丝含量的改变和其神经毒性有关。

Abstract:
Objective To investigate the alterations of cytoskeletal proteins in the cerebrum of 2, 5-hexanedione(HD) intoxicated rats.
Methods HD was administered by intraperitoneal injection to Wistar rats with daily dosing of 200 and 400 mg/kg for continuous 8 weeks(five times every week).Cerebrum tissues were homogenized and centrifuged at 28.000 g for 30 min to prepare the supernatant and pellet sample.The relative levels of light neurofilament(NF-L), midsized neurofilament(NF-M), heavy neurofilament(NF-H), β-actin, α-tubulin and β-tubulin were determined by SDS-PAGE and Western-Blot.
Results Excep that the contents of NF-L and NF-M couldn't be assayed for the reason of low concentration, HD intoxication resulted in a dose-dependent reduction of NF subunit proteins in the supernatant and pellet to 33%-73%.The contents of β-actin, α-tubulin and β-tubulin remained relatively unaffected in the pellet and the supernatant compared with the control group.
Conclusion HD induced alterations in cytoskeletal proteins in the cerebrum of rats, and the alterations were related to its peripheral and central neurotioxicity.

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