单细胞凝胶电泳检测酞酸酯类对DNA的损伤

赵文红; 厉曙光; 石红军

单细胞凝胶电泳检测酞酸酯类对DNA的损伤

Effect of DEHP on DNA damage of mice embryo fibroblast (3T6) using single cell gel electrophoresis

摘要

摘要:
目的应用单细胞凝胶电泳(SCGE)技术对增塑剂(DEHP)邻苯二甲酸二乙基己酯致小鼠胚胎成纤维细胞(3T6细胞)DNA损伤、损伤程度以及有无剂量-效应关系进行检测。
方法将溶剂二甲基亚砜处理的3T6细胞作为阴性对照组, 用H ₂O ₂染毒的3T6细胞作为阳性对照组(80μmol/LH ₂O ₂染毒), 将不同浓度DEHP处理的3T6细胞设为4个剂量组(62.5, 125, 250, 500μg/ml)进行SCGE检验。
结果各染毒组细胞DNA损伤与阴性对照组比较, 均有显著性差异(P < 0.05);且随DEHP染毒浓度增加, 3T6细胞DNA损伤程度加重, 有剂量-效应关系。
结论 DEHP体外染毒对3T6细胞能造成损伤, 且随剂量的增加有加重的趋势。

Abstract:
Objective To determinate the damage of DNA, the degree of DNA damage and the dose-effect of mice embryo fibroblast(3T6)caused by DEHP using single cell gel electrophor esis(SCGE).
Methods Using the mice embryo fibroblast not treated with DEHP as the negative control group, 80μmol/L H ₂O ₂ treated mice embryo fibroblast as the positive control group, the different concentration DEHP exposed to mice embryo fibroblast was as four dose groups(62.5, 125.0, 250.0, 500.0 μg/ml), DNA damage was detected by SCGE.
Results The different degree of DNA damage between groups treated with DEHP and the negative group was significant, and when the concentration of DEHP increased, the damage of the DNAin the mice embryo fibroblast was aggravated, having dose-effect.
Conclusion DEHP can cause DNA damage to mice embryo fibroblast and the degree of DNA damage was aggravated with the increase of DEHP.

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