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周建嫦, 黄俊明, 杨杏芬, 凌文华, 杨明杰, 黄琼, 李文立. PCR法筛选检测转基因食品[J]. 中国公共卫生, 2004, 20(10): 1183-1185.
引用本文: 周建嫦, 黄俊明, 杨杏芬, 凌文华, 杨明杰, 黄琼, 李文立. PCR法筛选检测转基因食品[J]. 中国公共卫生, 2004, 20(10): 1183-1185.
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ZHOU Jian-chang, HUANG Jun-ming, YANG Xing-fen, . Screening detection of genetically modified foods by polymerase chain reaction[J]. Chinese Journal of Public Health, 2004, 20(10): 1183-1185.
Citation: ZHOU Jian-chang, HUANG Jun-ming, YANG Xing-fen, . Screening detection of genetically modified foods by polymerase chain reaction[J]. Chinese Journal of Public Health, 2004, 20(10): 1183-1185.
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PCR法筛选检测转基因食品

Screening detection of genetically modified foods by polymerase chain reaction

    摘要
  • 摘要:
      目的   通过PCR检测花椰菜花叶病毒(camv)35S启动子和胭脂碱合酶(nos)终止子建立筛选食品中有无转基因成分的方法。
      方法   用改良溴化十六烷三甲基铵(CTAB)法制备转基因抗除草剂〔大豆RoundUp ReadyTMSoybean(RRS)〕和转基因抗虫玉米系列标准品Bt176 Maximaizer的DNA, PCR检测其内参照基因及camv35S启动子和nos终止子。
      结果   改良CTAB法制备的DNA用作PCR模板均可扩增出内参照基因, PCR扩增camv35S启动子可检测出含量为0.5%的RRS和Bt176 Maximaizer, 而PCR扩增nos终止子可检测出含1%RRS的食品样品。
      结论   改良CTAB法制备的DNA可用作PCR模板, 建立的PCR检测camv35S启动子和nos终止子方法可用于筛选食品中有无转基因成分。

     

    Abstract:
      Objective   To establish the screening method of detecting genetically modified food in food stuffs by PCR amplifying the camv 35 S promoter and nos terminator.
      Methods   The DNAs were extracted from genetically modified herbicide-tolerance soybean RoundUp ReadyTM Soybean(RRS)and genetically modified insect-resistance maize Bt176 Maximaizer using modified CTAB method, and then were used as templates to amplify the grain-specific endogenuous reference genes as well as camv 35 S promoter and nos terminator.
      Results   The g rain-specific endogenuous reference genes were amplified successfully when using DNAs extracted with modified CTAB method.0.5% RRS and 0.5% Bt 176 Maximaizer could be identified by PCR detecting camv 35 S promoter, and 1% RRS could be indentified by PCR detecting nos terminator.
      Conclusion   DNA extracted from genetically modified food by using modified CTAB methods can be used as template for PCR, the PCR method of detecting camv 35 S promoter and nos terminator can be used for screening genetically modified foods.

     

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